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Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats.
doi: 10.1073/pnas.1813956115
Figure Lengend Snippet: Fig. 4. Zip and ZnT expression profiles of Zn-deficient middle-aged rat prostates resemble profiles of human prostate cancer and the relationship between miR-182 up-regulation and ZIP1 mRNA/protein down-regulation. (A and B) qPCR analyses of Zn importers (Zip 1–14; Zip12 is not detectable) (A) and Zn exporters (ZnT 1–10) (B) in prostates from Zn-deficient vs. Zn-sufficient middle-aged Wistar-Unilever rats (Oaz1 as normalizer, measurements performed in triplicate, n = 9–11 rats per cohort; two-sided t test). Asterisks denote Zip and ZnT expression similarly up- or down-regulated in human prostate cancer. (C) ISH cellular localization of miR-182 by mmu-miR-182 detection probe (double digoxigenin-labeled at the 5′ and 3′ ends) and IHC analysis of ZIP1 protein expression in FFPE prostate tissues of Zn-deficient vs. Zn-sufficient middle-aged rat prostates. Intense/frequent miR-182 ISH signal (blue) was detected in Zn- deficient middle-aged prostate (two representative samples are shown) vs. very weak and diffuse miR-182 ISH signals (blue) in Zn-sufficient middle-aged prostate (blue, 4-nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyl phosphate counterstained by nuclear fast red). ZIP1 protein expression was diffuse and weak (brown staining, DAB) in representative ZN-deficient middle-aged rat prostate epithelia vs. strong ZIP1 expression (brown staining) in epithelial cells of a Zn-sufficient middle-aged rat prostate. n = 10 rats per group. (Scale bars: 50 μm in main panels and 25 μm in Insets.)
Article Snippet: Tissue sections were incubated with primary antibodies for mouse monoclonal PCNA (dilution 1:300, clone PC-10, Ab-1; Thermo Scientific), rabbit polyclonal NF-κΒ p65 (dilution 1:500, ab7970; Abcam), rabbit polyclonal COX-2 (dilution 1:300, NB1-689; Novus Biologicals), and
Techniques: Expressing, Labeling, Staining